Hotspot

trnI-GAU-rrn16

Genome · 6495-7343 · 849 bp

Back to Araucaria

Pi 0.0212

MarkerSeek score 70.50

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: Genome
length_bp: 849
variable_sites: 18
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	57.8	57.7	992-994 bp	1.0	77.8
trnI-GAU-rrn16_p2	CGCTGATGAGCACATTGAAC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	57.8	58.3	1014-1016 bp	1.0	77.7
trnI-GAU-rrn16_p3	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	941-943 bp	1.0	77.7
trnI-GAU-rrn16_p4	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	58.2	58.3	963-965 bp	1.0	77.6
trnI-GAU-rrn16_p5	CGCTGATGAGCACATTGAAC	AAGTCGTAACAAGGTAGCCG	50.0	50.0	57.8	57.6	1001-1003 bp	1.0	77.3