Hotspot

rrn5-rrn4.5

Genome · 196-632 · 437 bp

Back to Araucaria

Pi 0.0206

MarkerSeek score 67.20

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: Genome
length_bp: 437
variable_sites: 9
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	GCATCGAGCTATTTTTCCGC	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	613-614 bp	1.0	80.6
rrn5-rrn4.5_p2	GCATCGAGCTATTTTTCCGC	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	587-588 bp	1.0	80.6
rrn5-rrn4.5_p3	GCATCGAGCTATTTTTCCGC	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	585-586 bp	1.0	80.6
rrn5-rrn4.5_p4	AGTATCGTCACCGCAGTAGA	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	579-580 bp	1.0	80.6
rrn5-rrn4.5_p5	TATCGTCACCGCAGTAGAGT	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	577-578 bp	1.0	80.6