Hotspot

ycf1

SSC · 126128-131665 · 5538 bp

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Pi 0.0116
MarkerSeek score 52.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5538
variable_sites
289
pi
0.0116
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CCTTCTTGAATTACGCCCGA GATGTCCTTGCCCACACTAG 50.0 55.0 58.0 58.0 594-690 bp 0.947368 82.4
ycf1_p2 CCTTCTTGAATTACGCCCGA ACGAAGGGGACTAAACAGGA 50.0 50.0 58.0 58.0 1073-1169 bp 0.947368 80.6
ycf1_p3 TCTTGAATTACGCCCGACTG GATGTCCTTGCCCACACTAG 50.0 55.0 58.0 58.0 591-687 bp 0.894737 80.1
ycf1_p4 TCTTGAATTACGCCCGACTG ACGAAGGGGACTAAACAGGA 50.0 50.0 58.0 58.0 1070-1166 bp 0.894737 78.4
ycf1_p5 TCTTGAATTACGCCCGACTG ACAGGAACAAGAGGGATCCA 50.0 50.0 58.0 58.0 1056-1152 bp 0.894737 78.3