Hotspot

clpP

LSC · 71985-73982 · 1998 bp

Pi 0.0106

MarkerSeek score 52.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1998
variable_sites: 86
pi: 0.0106
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	AACAGGGTCAGAGGGATCAA	50.0	50.0	58.0	58.0	2834-2856 bp	1.0	83.6
clpP_p2	CTGAACGAGTCGCACATACA	ACAGGGTCAGAGGGATCAAA	50.0	50.0	58.0	58.0	2833-2855 bp	1.0	83.6
clpP_p3	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2866-2888 bp	1.0	83.4
clpP_p4	GATCTGAACGAGTCGCACAT	ACAGGGTCAGAGGGATCAAA	50.0	50.0	58.1	58.0	2836-2858 bp	1.0	83.1
clpP_p5	CTGAACGAGTCGCACATACA	GATTCGGTATAGCCCCTCCT	50.0	55.0	58.0	58.1	2943-2965 bp	0.789474	74.4