Hotspot

petA-psbJ

LSC · 64926-65945 · 1020 bp

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Pi 0.0147
MarkerSeek score 53.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1020
variable_sites
58
pi
0.0147
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.0 57.6 1163-1179 bp 1.0 80.9
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC ATTCCGGATTGGGTTCATCC 50.0 50.0 57.8 57.6 1146-1162 bp 1.0 80.0
petA-psbJ_p3 CATTACGTGTCCAAGGCCTT ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1160-1176 bp 1.0 79.8
petA-psbJ_p4 TTACGTGTCCAAGGCCTTTT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.6 57.6 1158-1174 bp 1.0 78.4
petA-psbJ_p5 TACGTGTCCAAGGCCTTTTG ATTCCGGATTGGGTTCATCC 50.0 50.0 58.4 57.6 1157-1173 bp 1.0 78.0