Hotspot

accD-psaI

LSC · 60969-61650 · 682 bp

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Pi 0.0118
MarkerSeek score 56.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
682
variable_sites
39
pi
0.0118
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCGGGTACTTGGGATCCTAT CTCCTTTATGTTCGGCGGAA 55.0 50.0 58.0 58.0 1582-1601 bp 1.0 83.8
accD-psaI_p2 CCGGGTACTTGGGATCCTAT TTGTTTTTCGAGACCCCGTT 55.0 45.0 58.0 58.0 1930-1949 bp 1.0 83.5
accD-psaI_p3 GACAGGATTAACTGAGGCCG CTCCTTTATGTTCGGCGGAA 55.0 50.0 58.1 58.0 1475-1494 bp 0.789474 74.9
accD-psaI_p4 CCGGGTACTTGGGATCCTAT GCCATACGTCGATCCTGATC 55.0 55.0 58.0 58.0 1677-1678 bp 0.157895 46.6
accD-psaI_p5 GACAGGATTAACTGAGGCCG GCCATACGTCGATCCTGATC 55.0 55.0 58.1 58.0 1570-1571 bp 0.157895 46.3