Hotspot

trnT-GGU-psbD

LSC · 33618-35021 · 1404 bp

Back to Aralia

Pi 0.011
MarkerSeek score 61.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1404
variable_sites
72
pi
0.011
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AACGGGCCCTTTTAACTCAG GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1547-1588 bp 1.0 84.2
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA GGACCAGCCTACAAAAACGA 50.0 50.0 57.9 58.1 1542-1583 bp 1.0 84.2
trnT-GGU-psbD_p3 AACGGGCCCTTTTAACTCAG ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1537-1578 bp 1.0 83.2
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1532-1573 bp 1.0 83.1
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1505-1546 bp 1.0 82.9