Hotspot

trnE-UUC-trnT-GGU

LSC · 32720-33545 · 826 bp

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Pi 0.0184
MarkerSeek score 70.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
826
variable_sites
49
pi
0.0184
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.94
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 883-930 bp 1.0 87.0
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 920-967 bp 1.0 86.9
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.8 57.9 848-895 bp 1.0 86.4
trnE-UUC-trnT-GGU_p4 TTTCGTAGTACCCTACCCCC TACCGCTGAGTTAAAAGGGC 55.0 50.0 57.8 57.9 885-932 bp 1.0 86.3
trnE-UUC-trnT-GGU_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 861-908 bp 1.0 86.2