Hotspot

trnC-GCA-petN

LSC · 29516-30198 · 683 bp

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Pi 0.0129
MarkerSeek score 57.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
683
variable_sites
39
pi
0.0129
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.1 58.0 736-810 bp 1.0 87.6
trnC-GCA-petN_p2 CGATATGGCCGAGTGGTAAG TTAAAGCAGCCCAAGCAAGA 55.0 45.0 57.9 58.0 776-850 bp 1.0 87.4
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 50.0 50.0 58.1 57.8 730-804 bp 1.0 86.1
trnC-GCA-petN_p4 CGATATGGCCGAGTGGTAAG CAGCCCAAGCAAGACTTACT 55.0 50.0 57.9 57.8 770-844 bp 1.0 86.0
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.4 58.0 754-828 bp 1.0 85.3