Hotspot

rpoB-trnC-GCA

LSC · 28162-29444 · 1283 bp

Back to Aralia

Pi 0.0115
MarkerSeek score 59.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1283
variable_sites
60
pi
0.0115
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCCCTCATTTCCATCTCGGA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.8 58.1 1184-1380 bp 1.0 85.6
rpoB-trnC-GCA_p2 TCCCTCATTTCCATCTCGGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.8 58.4 1166-1362 bp 1.0 83.4
rpoB-trnC-GCA_p3 TCCCTCATTTCCATCTCGGA GACACCCGGATTTGAACTGG 50.0 55.0 57.8 58.8 1189-1385 bp 1.0 79.5
rpoB-trnC-GCA_p4 TTCCCTCATTTCCATCTCGG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.0 58.1 1185-1381 bp 1.0 78.9
rpoB-trnC-GCA_p5 TTCCCTCATTTCCATCTCGG AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.0 58.4 1167-1363 bp 1.0 76.8