Hotspot

atpH-atpI

LSC · 14779-15867 · 1089 bp

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Pi 0.0106
MarkerSeek score 63.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1089
variable_sites
57
pi
0.0106
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.0 58.5 1016-1213 bp 1.0 83.4
atpH-atpI_p2 TACCTTGACCAACTCCAGGT TTTTTGCAACTTTAGCCGCG 50.0 45.0 57.9 58.5 1072-1269 bp 1.0 82.6
atpH-atpI_p3 AGCCAATCCAGCAGCAATAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 57.9 58.5 1032-1229 bp 1.0 82.5
atpH-atpI_p4 AATAACGGAAGCGGCAGAAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.2 58.5 1017-1214 bp 1.0 82.2
atpH-atpI_p5 GCAGTACCTTGACCAACTCC TTTTTGCAACTTTAGCCGCG 55.0 45.0 58.2 58.5 1076-1273 bp 1.0 82.0