Hotspot

trnS-GCU-trnG-UCC

LSC · 9438-10164 · 727 bp

Back to Aralia

Pi 0.0173
MarkerSeek score 64.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
727
variable_sites
57
pi
0.0173
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA ACAACGAATCACACTTTTACCAC 45.0 39.1 58.0 57.8 797-809 bp 1.0 61.7
trnS-GCU-trnG-UCC_p2 ACGGAAAGAGAGGGATTCGA ACAACGAATCACACTTTTACCAC 50.0 39.1 58.2 57.8 846-858 bp 1.0 60.4
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA CAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.6 796-808 bp 1.0 59.7
trnS-GCU-trnG-UCC_p4 ATTAGCAATCCGCCGCTTTA ACAACGAATCACACTTTTACCA 45.0 36.4 58.0 56.5 797-809 bp 1.0 59.0
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA CAACGAATCACACTTTTACCACT 50.0 39.1 58.2 57.6 845-857 bp 1.0 58.4