Hotspot

psbK-psbI

LSC · 8691-9100 · 410 bp

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Pi 0.0173
MarkerSeek score 60.10
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
410
variable_sites
24
pi
0.0173
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG CCCGGATCATTAGATAGGAATCC 50.0 47.8 57.9 57.7 522-527 bp 1.0 57.7
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT CCCGGATCATTAGATAGGAATCC 45.0 47.8 58.3 57.7 519-524 bp 1.0 55.7
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT CCCGGATCATTAGATAGGAATCC 45.0 47.8 58.3 57.7 520-525 bp 1.0 55.7
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT CCCGGATCATTAGATAGGAATCC 45.0 47.8 58.3 57.7 521-526 bp 1.0 55.7
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG ACGAAAAGTTTGAGAGTAAGCA 50.0 36.4 57.9 56.2 459-464 bp 1.0 54.6