Hotspot

trnK-UUU-rps16

LSC · 4356-5231 · 876 bp

Back to Aralia

Pi 0.017
MarkerSeek score 66.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
876
variable_sites
63
pi
0.017
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCTTCGCTTCATCCA AGAAGGCGGAGGTTTTTAAGT 50.0 42.9 58.1 57.5 961-998 bp 1.0 76.0
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AGAAGGCGGAGGTTTTTAAGT 55.0 42.9 57.9 57.5 974-1011 bp 1.0 75.8
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AGAAGGCGGAGGTTTTTAAGT 50.0 42.9 58.2 57.5 913-950 bp 1.0 75.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGAAGGCGGAGGTTTTTAAGT 50.0 42.9 58.2 57.5 912-949 bp 1.0 75.5
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC AGAAGGCGGAGGTTTTTAAGT 50.0 42.9 57.8 57.5 998-1035 bp 1.0 74.4