Hotspot

ycf1

SSC · 120804-126266 · 5463 bp

Pi 0.00128

MarkerSeek score 51.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5463
variable_sites: 7
pi: 0.00128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATTTGTACGACCACCGAGG	TCTAAATTCCGGATGGCATCC	50.0	47.6	57.9	57.9	198 bp	1.0	71.0
ycf1_p2	AATTTGTACGACCACCGAGG	CTAAATTCCGGATGGCATCC	50.0	50.0	57.9	56.3	197 bp	1.0	66.7
ycf1_p3	CGTTTGGCTTTTCTTGAACGA	TCTAAATTCCGGATGGCATCC	42.9	47.6	57.9	57.9	282 bp	1.0	63.0
ycf1_p4	ACGTTTGGCTTTTCTTGAACG	TCTAAATTCCGGATGGCATCC	42.9	47.6	58.2	57.9	283 bp	1.0	61.8
ycf1_p5	TAATTTGTACGACCACCGAGG	TCTAAATTCCGGATGGCATCC	47.6	47.6	57.8	57.9	199 bp	1.0	61.8