Hotspot

rpl16

LSC · 78949-80223 · 1275 bp

Back to Aragoa

Pi 0.00157
MarkerSeek score 59.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1275
variable_sites
2
pi
0.00157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCATTTCTCTTGGTTCTGCGT CTGTCCAATCAAAGCAAGCA 42.9 45.0 57.9 56.9 1380-1381 bp 1.0 62.4
rpl16_p2 TCATTTCTCTTGGTTCTGCGT CTGTCCAATCAAAGCAAGCAA 42.9 42.9 57.9 57.6 1380-1381 bp 1.0 60.1
rpl16_p3 TCATTTCTCTTGGTTCTGCGT TCTGTCCAATCAAAGCAAGC 42.9 45.0 57.9 56.6 1381-1382 bp 1.0 60.1
rpl16_p4 TCATTTCTCTTGGTTCTGCGT TCTGTCCAATCAAAGCAAGCA 42.9 42.9 57.9 58.4 1381-1382 bp 1.0 59.8
rpl16_p5 TCATTTCTCTTGGTTCTGCGT TGTCCAATCAAAGCAAGCAA 42.9 40.0 57.9 56.4 1379-1380 bp 1.0 58.6