Hotspot

ndhH

SSC · 118909-120090 · 1182 bp

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Pi 0.00169
MarkerSeek score 59.70
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
2
pi
0.00169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 ACGGATTCGAATCTGGAAAA TGCATGGAAGAATGACTCGG 40.0 50.0 55.1 58.0 1273 bp 1.0 56.5
ndhH_p2 ACGGATTCGAATCTGGAAAAAG TGCATGGAAGAATGACTCGG 40.9 50.0 57.1 58.0 1273 bp 1.0 56.3
ndhH_p3 ACGGATTCGAATCTGGAAAA CGTTCAGCAATGCATGGAAG 40.0 50.0 55.1 58.1 1283 bp 1.0 56.0
ndhH_p4 ACGGATTCGAATCTGGAAAA TACGTTCAGCAATGCATGGA 40.0 45.0 55.1 57.9 1285 bp 1.0 55.9
ndhH_p5 ACGGATTCGAATCTGGAAAAAG CGTTCAGCAATGCATGGAAG 40.9 50.0 57.1 58.1 1283 bp 1.0 55.8