Hotspot

ndhF

SSC · 107242-109482 · 2241 bp

Back to Aragoa

Pi 0.00178
MarkerSeek score 58.20
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2241
variable_sites
4
pi
0.00178
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACATATCCCCCTAGTGTATCGA CAAAAGAGGGAAGATCCGGC 45.5 55.0 57.3 58.6 2385 bp 1.0 53.8
ndhF_p2 TCCCCCTAGTGTATCGATTT CAAAAGAGGGAAGATCCGGC 45.0 55.0 55.0 58.6 2380 bp 1.0 51.3
ndhF_p3 ACATATCCCCCTAGTGTATCGA TCAAAAGAGGGAAGATCCGG 45.5 50.0 57.3 56.9 2386 bp 1.0 50.1
ndhF_p4 ACATATCCCCCTAGTGTATCGA CGGCGAATCGGAAACAATTA 45.5 45.0 57.3 56.9 2369 bp 1.0 50.0
ndhF_p5 TCCCCCTAGTGTATCGATTTT CAAAAGAGGGAAGATCCGGC 42.9 55.0 55.8 58.6 2380 bp 1.0 50.0