Hotspot

ndhD

SSC · 112610-114139 · 1530 bp

Pi 0

MarkerSeek score 26.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGAGTTCTTGCCGGTTGAAA	GATACCCTGGGACGGATGTA	45.0	55.0	58.0	58.0	1893 bp	1.0	80.0
ndhD_p2	GGAATTCTTTCTGGAGCGGT	GATACCCTGGGACGGATGTA	50.0	55.0	57.9	58.0	2269-2270 bp	1.0	79.4
ndhD_p3	CTAACGAAGCATGGGGATCC	GATACCCTGGGACGGATGTA	55.0	55.0	58.1	58.0	2244-2245 bp	1.0	79.4
ndhD_p4	TGAGTTCTTGCCGGTTGAAA	GATTTGATTTTGCGCACGGA	45.0	45.0	58.0	58.0	1650 bp	0.5	59.4
ndhD_p5	GGAATTCTTTCTGGAGCGGT	GATTTGATTTTGCGCACGGA	50.0	45.0	57.9	58.0	2027 bp	0.5	58.9