Hotspot

ndhD-psaC

SSC · 114140-114244 · 105 bp

Back to Aragoa

Pi 0.0952
MarkerSeek score 86.90
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
105
variable_sites
10
pi
0.0952
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 GAACCCGCAAATATTGGCAA ATGGCATGAAACAACCCGTA 45.0 45.0 57.3 57.8 228 bp 1.0 76.5
ndhD-psaC_p2 GAACCCGCAAATATTGGCAA TATGGCATGAAACAACCCGT 45.0 45.0 57.3 57.8 229 bp 1.0 76.5
ndhD-psaC_p3 GAACCCGCAAATATTGGCAA CCCGTAGTATGGGTCTAGCT 45.0 55.0 57.3 57.7 214 bp 1.0 76.1
ndhD-psaC_p4 TGCCCTTATGTGGAAGAAGG ATGGCATGAAACAACCCGTA 50.0 45.0 57.2 57.8 257 bp 1.0 75.0
ndhD-psaC_p5 GAACCCGCAAATATTGGCAA GTAAGAGGTGTGAATCCGCC 45.0 55.0 57.3 58.3 283 bp 1.0 74.7