Hotspot

petA-psbJ

LSC · 61306-62230 · 925 bp

Back to Aragoa

Pi 0.00757
MarkerSeek score 63.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
925
variable_sites
7
pi
0.00757
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA GCTGGTATTCTTGTGATCGGT 50.0 47.6 58.2 58.1 1033 bp 1.0 70.4
petA-psbJ_p2 GAGAAGGTTCAATTGGCCGA AGCTGGTATTCTTGTGATCGG 50.0 47.6 58.2 57.8 1034 bp 1.0 69.6
petA-psbJ_p3 AGAAGGTTCAATTGGCCGAA GCTGGTATTCTTGTGATCGGT 45.0 47.6 57.7 58.1 1032 bp 1.0 69.5
petA-psbJ_p4 TGGGCTCTGTTATTTTGGCA GCTGGTATTCTTGTGATCGGT 45.0 47.6 57.7 58.1 1086 bp 1.0 69.4
petA-psbJ_p5 AGAAGGTTCAATTGGCCGAA AGCTGGTATTCTTGTGATCGG 45.0 47.6 57.7 57.8 1033 bp 1.0 68.8