Hotspot

ndhK-ndhC

LSC · 48045-48097 · 53 bp

Back to Aragoa

Pi 0.0189
MarkerSeek score 63.30
Primer available yes
Label ndhK-ndhC

Feature Metadata

label_name
ndhK-ndhC
feature_type
igs
region
LSC
length_bp
53
variable_sites
1
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK-ndhC_p1 ACCATAGAGAAGGGGCCATA TTTATGCATGGCGAAAGGGA 50.0 45.0 57.2 57.9 211 bp 1.0 72.8
ndhK-ndhC_p2 ACCATAGAGAAGGGGCCATA TGGGCAATGAGTTTCGATGT 50.0 45.0 57.2 57.8 293 bp 1.0 72.2
ndhK-ndhC_p3 ACCATAGAGAAGGGGCCATA GTTTATGCATGGCGAAAGGG 50.0 50.0 57.2 57.8 212 bp 1.0 72.2
ndhK-ndhC_p4 ACCATAGAGAAGGGGCCATA TTATGCATGGCGAAAGGGAG 50.0 50.0 57.2 58.3 210 bp 1.0 71.3
ndhK-ndhC_p5 ACCATAGAGAAGGGGCCATAA TTTATGCATGGCGAAAGGGA 47.6 45.0 57.9 57.9 211 bp 1.0 70.7