Hotspot

trnT-UGU-trnL-UAA

LSC · 44652-45304 · 653 bp

Back to Aragoa

Pi 0.00306
MarkerSeek score 53.50
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
653
variable_sites
2
pi
0.00306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 714 bp 1.0 77.2
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 708 bp 1.0 75.9
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG GGTTTCCATACCAAGGCTCA 55.0 50.0 57.9 57.5 746 bp 1.0 75.1
trnT-UGU-trnL-UAA_p4 CTAACCTCTGAGCTAAGCGG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.5 58.2 706 bp 1.0 74.2
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG CGTAGCGTCTACCGATTTCG 55.0 55.0 57.9 58.6 713 bp 1.0 74.2