Hotspot

trnT-GGU-psbD

LSC · 30153-31410 · 1258 bp

Back to Aragoa

Pi 0.00238
MarkerSeek score 60.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1258
variable_sites
3
pi
0.00238
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GACCAGCCTACAAACACGAA 55.0 50.0 58.1 58.1 1417 bp 1.0 78.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GACCAGCCTACAAACACGAA 50.0 50.0 58.1 58.1 1420 bp 1.0 78.5
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG GAAGAGCAATAGACCGGACC 55.0 55.0 58.1 57.8 1433 bp 1.0 77.8
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG GGAAGAGCAATAGACCGGAC 55.0 55.0 58.1 57.8 1434 bp 1.0 77.8
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA GAAGAGCAATAGACCGGACC 50.0 55.0 58.1 57.8 1436 bp 1.0 77.7