Hotspot

rpoC2

LSC · 41810-45949 · 4140 bp

Back to Arachis

Pi 0.00462

MarkerSeek score 42.80

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4140
variable_sites: 65
pi: 0.00462
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AGGATCTTCAAATTCCAATTAAGA	TATGTTGCTGCATGGATCGG	29.2	50.0	54.2	58.4	218 bp	1.0	51.0
rpoC2_p2	AAAAGGATCTTCAAATTCCAAT	TATGTTGCTGCATGGATCGG	27.3	50.0	52.2	58.4	221 bp	1.0	50.9
rpoC2_p3	AAAGGATCTTCAAATTCCAATT	TATGTTGCTGCATGGATCGG	27.3	50.0	52.2	58.4	220 bp	1.0	50.9
rpoC2_p4	AAAAGGATCTTCAAATTCCAATT	TATGTTGCTGCATGGATCGG	26.1	50.0	53.1	58.4	221 bp	1.0	50.9
rpoC2_p5	AAAAAGGATCTTCAAATTCCAA	TATGTTGCTGCATGGATCGG	27.3	50.0	52.4	58.4	222 bp	1.0	50.8