Hotspot

rpl16-rps3

LSC · 83187-84404 · 1218 bp

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Pi 0.00502

MarkerSeek score 59.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 31
pi: 0.00502
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTCAAGTGCCTGAAGAGCA	TCCTCTACAAACCATTCGAGC	50.0	47.6	58.4	57.8	1441-1453 bp	1.0	69.2
rpl16-rps3_p2	GTTCAAGTGCCTGAAGAGCA	CCTCTACAAACCATTCGAGCT	50.0	47.6	58.4	57.7	1440-1452 bp	1.0	69.1
rpl16-rps3_p3	GGGTTCAAGTGCCTGAAGAG	TCCTCTACAAACCATTCGAGC	55.0	47.6	58.5	57.8	1443-1455 bp	1.0	68.6
rpl16-rps3_p4	GGGTTCAAGTGCCTGAAGAG	CCTCTACAAACCATTCGAGCT	55.0	47.6	58.5	57.7	1442-1454 bp	1.0	68.6
rpl16-rps3_p5	TTCAAGTGCCTGAAGAGCAT	TCCTCTACAAACCATTCGAGC	45.0	47.6	57.4	57.8	1440-1452 bp	1.0	67.8