Hotspot

rps11-rpl36

LSC · 80340-80913 · 574 bp

Back to Arachis

Pi 0.00717

MarkerSeek score 51.60

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 574
variable_sites: 20
pi: 0.00717
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTTTACGCGAACC	CCAAGACATAAACAAAGACAAGGA	50.0	37.5	58.1	57.4	503-651 bp	1.0	48.4
rps11-rpl36_p2	CGTCCATTTTTACGCGAACC	TCCAAGACATAAACAAAGACAAGG	50.0	37.5	58.1	57.4	504-652 bp	1.0	48.4
rps11-rpl36_p3	CGTCCATTTTTACGCGAACC	TGTTCCAATCCAAGACATAAACA	50.0	34.8	58.1	56.4	512-660 bp	1.0	48.4
rps11-rpl36_p4	CGTCCATTTTTACGCGAACC	TGTTCCAATCCAAGACATAAACAA	50.0	33.3	58.1	57.0	512-660 bp	1.0	46.5
rps11-rpl36_p5	CGTCCATTTTTACGCGAACC	TTGTTCCAATCCAAGACATAAACA	50.0	33.3	58.1	57.0	513-661 bp	1.0	46.5