Hotspot

trnP-UGG-psaJ

LSC · 67239-67673 · 435 bp

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Pi 0.00568
MarkerSeek score 51.40
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
435
variable_sites
13
pi
0.00568
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 512-544 bp 1.0 83.3
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 511-543 bp 1.0 83.3
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 523-555 bp 1.0 83.1
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 522-554 bp 1.0 83.1
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 525-557 bp 1.0 83.1