Hotspot

trnQ-UUG-accD

LSC · 56684-58069 · 1386 bp

Back to Arachis

Pi 0.00731

MarkerSeek score 52.80

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: LSC
length_bp: 1386
variable_sites: 46
pi: 0.00731
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	TTGGGTCTTCACTTGCACTG	55.0	50.0	58.1	58.3	1470-1537 bp	1.0	82.9
trnQ-UUG-accD_p2	GAGGTTCGAATCCTTCCGTC	ATTTCCGCCCTTACCACAAA	55.0	45.0	58.1	57.7	1928-1995 bp	1.0	81.9
trnQ-UUG-accD_p3	GAGGTTCGAATCCTTCCGTC	TTTCCGCCCTTACCACAAAT	55.0	45.0	58.1	57.7	1927-1994 bp	1.0	81.9
trnQ-UUG-accD_p4	TAAGGCATCGGGTTTTGGTC	ATTTCCGCCCTTACCACAAA	50.0	45.0	58.2	57.7	1958-2025 bp	1.0	81.1
trnQ-UUG-accD_p5	TAAGGCATCGGGTTTTGGTC	TTTCCGCCCTTACCACAAAT	50.0	45.0	58.2	57.7	1957-2024 bp	1.0	81.1