Hotspot

trnR-UCU-trnS-GCU

LSC · 53254-55035 · 1782 bp

Back to Arachis

Pi 0.00711

MarkerSeek score 54.30

Primer available yes

Label trnR-UCU-trnS-GCU

Feature Metadata

label_name: trnR-UCU-trnS-GCU
feature_type: igs
region: LSC
length_bp: 1782
variable_sites: 45
pi: 0.00711
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnS-GCU_p1	AGAAGACCTCTGTCCTATCCA	CAATCCGACGCTTTAGTCCA	47.6	50.0	56.9	58.0	1822-1879 bp	1.0	67.9
trnR-UCU-trnS-GCU_p2	AGAAGACCTCTGTCCTATCCA	AATCCGACGCTTTAGTCCAC	47.6	50.0	56.9	58.0	1821-1878 bp	1.0	67.9
trnR-UCU-trnS-GCU_p3	AGGTTTAGAAGACCTCTGTCCT	CAATCCGACGCTTTAGTCCA	45.5	50.0	57.9	58.0	1828-1885 bp	1.0	67.2
trnR-UCU-trnS-GCU_p4	AGGTTTAGAAGACCTCTGTCCT	AATCCGACGCTTTAGTCCAC	45.5	50.0	57.9	58.0	1827-1884 bp	1.0	67.2
trnR-UCU-trnS-GCU_p5	AGAAGACCTCTGTCCTATCCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	56.9	58.2	1875-1932 bp	1.0	66.6