Hotspot

trnC-GCA-rpoB

LSC · 34380-35582 · 1203 bp

Back to Arachis

Pi 0.00774

MarkerSeek score 53.40

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 38
pi: 0.00774
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	GACATTCCCGCATTTTCACC	50.0	50.0	58.1	57.7	1288-1310 bp	1.0	80.1
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	TAAGCCCCGATCAATGAACC	50.0	50.0	58.1	57.7	1352-1374 bp	1.0	79.4
trnC-GCA-rpoB_p3	ATTCTCTTGTCGATCAGGCG	GACATTCCCGCATTTTCACC	50.0	50.0	57.8	57.7	1312-1334 bp	1.0	79.0
trnC-GCA-rpoB_p4	CCGGATTTGAACTGGGGAAA	GCCCCGATCAATGAACCTAC	50.0	55.0	58.1	58.4	1349-1371 bp	1.0	78.9
trnC-GCA-rpoB_p5	ATTCTCTTGTCGATCAGGCG	TAAGCCCCGATCAATGAACC	50.0	50.0	57.8	57.7	1376-1398 bp	1.0	78.3