Hotspot

ycf3-psaA

LSC · 18620-19575 · 956 bp

Back to Arachis

Pi 0.0062

MarkerSeek score 58.20

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 23
pi: 0.0062
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	ATTGCTGAGGCTTGGTTTGA	TGCCCATTCCTCGAAAGAAG	45.0	50.0	58.0	57.9	1104-1151 bp	1.0	84.8
ycf3-psaA_p2	TTGCTGAGGCTTGGTTTGAT	TGCCCATTCCTCGAAAGAAG	45.0	50.0	58.0	57.9	1103-1150 bp	1.0	84.8
ycf3-psaA_p3	ATTGCTGAGGCTTGGTTTGA	GCCCATTCCTCGAAAGAAGT	45.0	50.0	58.0	57.9	1103-1150 bp	1.0	84.8
ycf3-psaA_p4	TTGCTGAGGCTTGGTTTGAT	GCCCATTCCTCGAAAGAAGT	45.0	50.0	58.0	57.9	1102-1149 bp	1.0	84.8
ycf3-psaA_p5	TTGGTTGAAGATCACAGGGC	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.1	57.9	1018-1065 bp	1.0	84.2