Hotspot

trnL-UAA-trnT-UGU

LSC · 14244-15146 · 903 bp

Back to Arachis

Pi 0.011

MarkerSeek score 54.10

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 38
pi: 0.011
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	TAGCGTCTACCAATTTCGCC	AAGAGAAAAGCCGGCTATCG	50.0	50.0	58.1	58.1	1010-1036 bp	1.0	82.5
trnL-UAA-trnT-UGU_p2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.1	57.9	959-985 bp	1.0	82.1
trnL-UAA-trnT-UGU_p3	AGTCCGTAGCGTCTACCAAT	AAGAGAAAAGCCGGCTATCG	50.0	50.0	58.2	58.1	1016-1042 bp	1.0	81.1
trnL-UAA-trnT-UGU_p4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	50.0	50.0	58.1	57.8	991-1017 bp	1.0	81.1
trnL-UAA-trnT-UGU_p5	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.2	57.9	965-991 bp	1.0	80.7