Hotspot

trnK-UUU-rbcL

LSC · 4357-5108 · 752 bp

Back to Arachis

Pi 0.00621
MarkerSeek score 60.70
Primer available yes
Label trnK-UUU-rbcL

Feature Metadata

label_name
trnK-UUU-rbcL
feature_type
igs
region
LSC
length_bp
752
variable_sites
23
pi
0.00621
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rbcL_p1 ACCCTACCGAGGTTATGGAC AGTCCTGAATTAGCTGCTGC 55.0 50.0 58.2 58.0 917-935 bp 1.0 82.4
trnK-UUU-rbcL_p2 ACCCTACCGAGGTTATGGAC CGAGCAAATGGAGTCCTGAA 55.0 50.0 58.2 57.9 928-946 bp 1.0 81.8
trnK-UUU-rbcL_p3 CCTACCGAGGTTATGGACGA AGTCCTGAATTAGCTGCTGC 55.0 50.0 58.3 58.0 915-933 bp 1.0 81.6
trnK-UUU-rbcL_p4 CCGAGGTTATGGACGAAACA AGTCCTGAATTAGCTGCTGC 50.0 50.0 57.6 58.0 911-929 bp 1.0 81.2
trnK-UUU-rbcL_p5 ACCGAGGTTATGGACGAAAC AGTCCTGAATTAGCTGCTGC 50.0 50.0 57.6 58.0 912-930 bp 1.0 81.1