Hotspot

ycf1

SSC · 122939-128278 · 5340 bp

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Pi 0.00639
MarkerSeek score 46.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5340
variable_sites
130
pi
0.00639
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TTGGTATGACCCACGGTTTC CTTGGATTGCATGGGCTAGA 50.0 50.0 57.8 57.6 627-633 bp 1.0 79.9
ycf1_p2 TTGGTATGACCCACGGTTTC AATCGTTGTAACAAACCGCG 50.0 45.0 57.8 57.7 1216-1222 bp 1.0 79.3
ycf1_p3 AAGCTCTCCTTGACCTACCA AATCGTTGTAACAAACCGCG 50.0 45.0 57.7 57.7 1371-1377 bp 0.875 70.8
ycf1_p4 AGCTCTCCTTGACCTACCAA AATCGTTGTAACAAACCGCG 50.0 45.0 57.7 57.7 1370-1376 bp 0.875 70.8
ycf1_p5 AGCTCTCCTTGACCTACCAA CTTGGATTGCATGGGCTAGA 50.0 50.0 57.7 57.6 781-787 bp 0.875 70.6