Hotspot

ndhF-rpl32

SSC · 111407-112221 · 815 bp

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Pi 0.0156
MarkerSeek score 54.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
815
variable_sites
39
pi
0.0156
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAAAGTCCACATACGCCGAA GGAGAAATAAGAACCTCCCCG 45.0 52.4 57.8 57.8 1251-1273 bp 1.0 73.6
ndhF-rpl32_p2 AAAGTCCACATACGCCGAAG GGAGAAATAAGAACCTCCCCG 50.0 52.4 58.3 57.8 1250-1272 bp 1.0 72.8
ndhF-rpl32_p3 AGAAAAGTCCACATACGCCG GGAGAAATAAGAACCTCCCCG 50.0 52.4 58.3 57.8 1253-1275 bp 1.0 72.8
ndhF-rpl32_p4 GAAAAGTCCACATACGCCGA GGAGAAATAAGAACCTCCCCG 50.0 52.4 58.3 57.8 1252-1274 bp 1.0 72.8
ndhF-rpl32_p5 GGTCGTGTGAACCAAAAACC GGAGAAATAAGAACCTCCCCG 50.0 52.4 57.8 57.8 1631-1653 bp 1.0 72.5