Hotspot

psaJ-rpl33

LSC · 65570-65965 · 396 bp

Back to Arabis

Pi 0.0177
MarkerSeek score 59.70
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
396
variable_sites
23
pi
0.0177
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 GATGCATTAACATTTCCCTTT TTTCGAACGCAACTGGTACA 33.3 45.0 52.7 58.1 487-530 bp 1.0 45.2
psaJ-rpl33_p2 GATGCATTAACATTTCCCTTT TCGAACGCAACTGGTACATT 33.3 45.0 52.7 57.8 485-528 bp 1.0 45.2
psaJ-rpl33_p3 GATGCATTAACATTTCCCTTT TTCGAACGCAACTGGTACAT 33.3 45.0 52.7 57.8 486-529 bp 1.0 45.2
psaJ-rpl33_p4 GATGCATTAACATTTCCCTTTT TTTCGAACGCAACTGGTACA 31.8 45.0 53.6 58.1 487-530 bp 1.0 45.2
psaJ-rpl33_p5 GATGCATTAACATTTCCCTTT ACGCAACTGGTACATTCCAA 33.3 45.0 52.7 57.7 481-524 bp 1.0 45.2