Hotspot

atpB-rbcL

LSC · 53120-53947 · 828 bp

Back to Arabis

Pi 0.00802
MarkerSeek score 54.10
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
828
variable_sites
24
pi
0.00802
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GCCGACTGGAACACTTAGAG CGGTCTCTCCAACGCATAAA 55.0 50.0 58.0 58.0 1784-1795 bp 1.0 82.0
atpB-rbcL_p2 CACCATGAGCTTTGGCAATG CGGTCTCTCCAACGCATAAA 50.0 50.0 58.0 58.0 2058-2069 bp 1.0 81.8
atpB-rbcL_p3 GCCGACTGGAACACTTAGAG GTACCCGCAGTAGCATTCAA 55.0 50.0 58.0 58.0 1871-1882 bp 1.0 81.8
atpB-rbcL_p4 CGGTTCATTCATCTGACCGT CGGTCTCTCCAACGCATAAA 50.0 50.0 58.0 58.0 2201-2212 bp 1.0 81.7
atpB-rbcL_p5 ATCTGACCGTAGACTAGGGC CGGTCTCTCCAACGCATAAA 55.0 50.0 58.0 58.0 2191-2202 bp 1.0 81.7