Hotspot

psbZ-trnG

LSC · 35182-35688 · 507 bp

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Pi 0.0134
MarkerSeek score 60.90
Primer available yes
Label psbZ-trnG

Feature Metadata

label_name
psbZ-trnG
feature_type
igs
region
LSC
length_bp
507
variable_sites
23
pi
0.0134
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG_p1 GTATTTGCGTCTCCTGAGGG CGGATAGCGGGAATCGAAC 55.0 57.9 58.1 58.2 671-690 bp 1.0 75.8
psbZ-trnG_p2 GTATTTGCGTCTCCTGAGGG GATAGCGGGAATCGAACCC 55.0 57.9 58.1 57.8 669-688 bp 1.0 75.7
psbZ-trnG_p3 GTATTTGCGTCTCCTGAGGG GGATAGCGGGAATCGAACC 55.0 57.9 58.1 57.8 670-689 bp 1.0 75.7
psbZ-trnG_p4 CCGTTGTATTTGCGTCTCCT CGGATAGCGGGAATCGAAC 50.0 57.9 58.3 58.2 676-695 bp 1.0 74.1
psbZ-trnG_p5 TGAGGGTTGGTCGAGTAACA CGGATAGCGGGAATCGAAC 50.0 57.9 58.3 58.2 657-676 bp 1.0 74.1