Hotspot

trnT-psbD

LSC · 31038-31938 · 901 bp

Back to Arabis

Pi 0.00852
MarkerSeek score 58.70
Primer available yes
Label trnT-psbD

Feature Metadata

label_name
trnT-psbD
feature_type
igs
region
LSC
length_bp
901
variable_sites
28
pi
0.00852
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-psbD_p1 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 1055-1242 bp 1.0 83.4
trnT-psbD_p2 TCAGTGGTAGAGTAACGCCA ACCTACAAAAACGAAGCGGT 50.0 45.0 58.1 58.0 1058-1245 bp 1.0 83.2
trnT-psbD_p3 GGCGTAAGTCATCGGTTCAA ACCTACAAAAACGAAGCGGT 50.0 45.0 58.3 58.0 1032-1219 bp 1.0 81.7
trnT-psbD_p4 TGGTAGAGTAACGCCATGGT ACCTACAAAAACGAAGCGGT 50.0 45.0 58.4 58.0 1054-1241 bp 1.0 80.4
trnT-psbD_p5 GTGGTAGAGTAACGCCATGG TCCCTCCGTAACCAGTCATC 55.0 55.0 58.1 58.5 1036-1223 bp 1.0 79.6