Hotspot

trnE-trnT

LSC · 30527-30965 · 439 bp

Back to Arabis

Pi 0.0105
MarkerSeek score 58.30
Primer available yes
Label trnE-trnT

Feature Metadata

label_name
trnE-trnT
feature_type
igs
region
LSC
length_bp
439
variable_sites
15
pi
0.0105
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-trnT_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 570-582 bp 1.0 82.5
trnE-trnT_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 549-561 bp 1.0 82.2
trnE-trnT_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 546-558 bp 1.0 82.1
trnE-trnT_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 532-544 bp 1.0 82.0
trnE-trnT_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 511-523 bp 1.0 82.0