Hotspot

trnS-trnG

LSC · 8013-8730 · 718 bp

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Pi 0.00992
MarkerSeek score 57.80
Primer available yes
Label trnS-trnG

Feature Metadata

label_name
trnS-trnG
feature_type
igs
region
LSC
length_bp
718
variable_sites
26
pi
0.00992
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-trnG_p1 CTTTAGTCCGCTCAGCCATC TACCACTAAACTACACCCGC 55.0 50.0 58.4 57.0 716-768 bp 1.0 75.1
trnS-trnG_p2 GCAATCCAACGCTTTAGTCC TACCACTAAACTACACCCGC 50.0 50.0 57.5 57.0 727-779 bp 1.0 74.1
trnS-trnG_p3 CTTTAGTCCGCTCAGCCATC TTACCACTAAACTACACCCGC 55.0 47.6 58.4 57.7 717-769 bp 1.0 72.8
trnS-trnG_p4 TTTAGTCCGCTCAGCCATCT TACCACTAAACTACACCCGC 50.0 50.0 58.8 57.0 715-767 bp 1.0 72.0
trnS-trnG_p5 CAATCCAACGCTTTAGTCCG TACCACTAAACTACACCCGC 50.0 50.0 57.2 57.0 726-778 bp 1.0 72.0