Hotspot

trnK-rps16

LSC · 4454-5139 · 686 bp

Back to Arabis

Pi 0.0137
MarkerSeek score 51.80
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
686
variable_sites
37
pi
0.0137
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 CCTTTCAGGATCAGTCGTGG CCGTTTATCGAATCGTTGCG 55.0 50.0 58.0 58.0 985-999 bp 1.0 85.8
trnK-rps16_p2 TCGACACCGAAAACAAGGTT CCGTTTATCGAATCGTTGCG 45.0 50.0 58.0 58.0 1078-1092 bp 1.0 85.5
trnK-rps16_p3 TCAGGATCAGTCGTGGTCTT CCGTTTATCGAATCGTTGCG 50.0 50.0 58.1 58.0 981-995 bp 1.0 85.3
trnK-rps16_p4 TTCAGGATCAGTCGTGGTCT CCGTTTATCGAATCGTTGCG 50.0 50.0 58.1 58.0 982-996 bp 1.0 85.3
trnK-rps16_p5 CCTTTCAGGATCAGTCGTGG AGGCAAAAGCCTCGTATACG 55.0 50.0 58.0 58.1 1061 bp 0.875 75.5