Hotspot

trnH-psbA

LSC · 75-473 · 399 bp

Back to Arabis

Pi 0.0188
MarkerSeek score 77.20
Primer available yes
Label trnH-psbA

Feature Metadata

label_name
trnH-psbA
feature_type
igs
region
LSC
length_bp
399
variable_sites
28
pi
0.0188
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-psbA_p1 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 446-499 bp 1.0 87.3
trnH-psbA_p2 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 461-514 bp 1.0 87.0
trnH-psbA_p3 ACTGCCTTAATCCACTTGGC GCTGTTGAGGCTCCATCTAC 50.0 55.0 58.2 58.1 447-500 bp 1.0 87.0
trnH-psbA_p4 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 464-517 bp 1.0 86.9
trnH-psbA_p5 ACTGCCTTAATCCACTTGGC CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.2 57.9 462-515 bp 1.0 86.8