Hotspot

ycf1

SSC · 124022-129382 · 5361 bp

Back to Arabidopsis

Pi 0.00593

MarkerSeek score 59.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5361
variable_sites: 203
pi: 0.00593
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAACGAGTCGGCCTCAAAAA	AGTTTGGGGAATGGAAACCG	45.0	50.0	57.5	58.4	1186-1198 bp	0.958333	77.8
ycf1_p2	TAACGAGTCGGCCTCAAAAA	GTTTGGGGAATGGAAACCGA	45.0	50.0	57.5	58.4	1185-1197 bp	0.958333	77.8
ycf1_p3	TAACGAGTCGGCCTCAAAAA	TTGGGGAATGGAAACCGAAC	45.0	50.0	57.5	58.4	1183-1195 bp	0.958333	77.8
ycf1_p4	TAACGAGTCGGCCTCAAAAA	TTTGGGGAATGGAAACCGAA	45.0	45.0	57.5	57.6	1184-1196 bp	0.958333	77.7
ycf1_p5	TAACGAGTCGGCCTCAAAAA	TTCAATTTCCCGAGTGGTCC	45.0	50.0	57.5	57.8	1433-1448 bp	0.916667	76.7