Hotspot

rpl32-trnL

SSC · 113708-114449 · 742 bp

Back to Arabidopsis

Pi 0.0118

MarkerSeek score 70.90

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 742
variable_sites: 58
pi: 0.0118
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	774-943 bp	1.0	74.3
rpl32-trnL_p2	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	767-936 bp	1.0	73.8
rpl32-trnL_p3	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	773-942 bp	1.0	73.7
rpl32-trnL_p4	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	772-941 bp	1.0	73.7
rpl32-trnL_p5	GGAAGGGATATTGGACATCGT	GAGCAGCGTGTCTACCAATT	47.6	50.0	57.2	58.3	768-937 bp	1.0	73.1