Hotspot

ndhF-rpl32

SSC · 112697-113548 · 852 bp

Back to Arabidopsis

Pi 0.017

MarkerSeek score 73.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 852
variable_sites: 77
pi: 0.017
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAAGGGA	ACGATGTCCAATATCCCTTCC	47.6	47.6	57.6	57.2	870-1031 bp	1.0	64.6
ndhF-rpl32_p2	TGGAAGTGGAATGAAAGGGA	ACGATGTCCAATATCCCTTCC	45.0	47.6	56.3	57.2	869-1030 bp	1.0	62.8
ndhF-rpl32_p3	CTGGAAGTGGAATGAAAGGGA	CGATGTCCAATATCCCTTCCT	47.6	47.6	57.6	56.9	869-1030 bp	1.0	62.3
ndhF-rpl32_p4	CTGGAAGTGGAATGAAAGGGA	AACGATGTCCAATATCCCTTCC	47.6	45.5	57.6	57.9	871-1032 bp	1.0	62.0
ndhF-rpl32_p5	CTGGAAGTGGAATGAAAGGGA	AAGCTTTCAACGATGTCCAA	47.6	40.0	57.6	55.9	879-1040 bp	1.0	61.7