Pi
0.00727
MarkerSeek score
66.00
Primer available
yes
Label
clpP-clpP
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| clpP-clpP_p1 | AAATTCTCCCGTTTGTGCCT | GCATCCTTTATCCTGGTCGG | 45.0 | 55.0 | 58.0 | 58.1 | 625-683 bp | 1.0 | 85.8 |
| clpP-clpP_p2 | AAATTCTCCCGTTTGTGCCT | AATTTGTGCGACCCGATGTA | 45.0 | 45.0 | 58.0 | 57.9 | 678-736 bp | 1.0 | 85.7 |
| clpP-clpP_p3 | CATAAAACGAGCTAGCGGGT | GCATCCTTTATCCTGGTCGG | 50.0 | 55.0 | 58.1 | 58.1 | 605-663 bp | 1.0 | 85.4 |
| clpP-clpP_p4 | CATAAAACGAGCTAGCGGGT | AATTTGTGCGACCCGATGTA | 50.0 | 45.0 | 58.1 | 57.9 | 658-716 bp | 1.0 | 85.3 |
| clpP-clpP_p5 | CTCCCGTTTGTGCCTCATAA | AATTTGTGCGACCCGATGTA | 50.0 | 45.0 | 57.9 | 57.9 | 673-731 bp | 1.0 | 84.9 |
| clpP-clpP_p1 | TTCGGTATCAACCTCTTGGC | TGGCGTTCCAAAAGTACCTT | 50.0 | 45.0 | 57.6 | 57.6 | 985-1021 bp | 1.0 | 81.0 |
| clpP-clpP_p2 | TTCGGTATCAACCTCTTGGC | ACCTTTTCGAAGTCCTGGAG | 50.0 | 50.0 | 57.6 | 57.2 | 970-1006 bp | 1.0 | 77.2 |
| clpP-clpP_p3 | CGGTATCAACCTCTTGGCC | TGGCGTTCCAAAAGTACCTT | 57.9 | 45.0 | 57.9 | 57.6 | 983-1019 bp | 1.0 | 75.6 |
| clpP-clpP_p4 | TTCGGTATCAACCTCTTGGC | CCTTTTCGAAGTCCTGGAGA | 50.0 | 50.0 | 57.6 | 56.9 | 969-1005 bp | 1.0 | 74.9 |
| clpP-clpP_p5 | TTTCGGTATCAACCTCTTGGC | TGGCGTTCCAAAAGTACCTT | 47.6 | 45.0 | 58.3 | 57.6 | 986-1022 bp | 1.0 | 73.9 |