Hotspot

rpoB-trnC

LSC · 26210-27307 · 1098 bp

Back to Arabidopsis

Pi 0.00936

MarkerSeek score 63.50

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1098
variable_sites: 71
pi: 0.00936
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	CCGGGTATTGTAGATGTTCCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	57.9	58.1	1146-1216 bp	1.0	79.2
rpoB-trnC_p2	GTTCCCTCTTTTTCATCCCCA	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.8	58.1	1131-1201 bp	1.0	78.8
rpoB-trnC_p3	TGTTCCCTCTTTTTCATCCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.8	58.1	1132-1202 bp	1.0	78.8
rpoB-trnC_p4	CCTCTTTTTCATCCCCAAGC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.7	58.1	1127-1197 bp	1.0	77.5
rpoB-trnC_p5	CCGGGTATTGTAGATGTTCCC	AAAAGGATTTGCAGTCCCCC	52.4	50.0	57.9	58.4	1128-1198 bp	1.0	77.1