Hotspot

trnS-trnG

LSC · 7831-8484 · 654 bp

Back to Arabidopsis

Pi 0.0183

MarkerSeek score 75.00

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 654
variable_sites: 64
pi: 0.0183
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	CCAACGCTTTAGTCCACTCA	AACCGAGAGACCCTTTAACT	50.0	45.0	57.8	55.8	692-878 bp	1.0	71.7
trnS-trnG_p2	AACGCTTTAGTCCACTCAGC	AACCGAGAGACCCTTTAACT	50.0	45.0	58.2	55.8	690-876 bp	1.0	71.4
trnS-trnG_p3	GAGAAGGGGAAAGAGAGGGA	AACCGAGAGACCCTTTAACT	55.0	45.0	57.7	55.8	755-941 bp	1.0	70.7
trnS-trnG_p4	AGGAGAAGGGGAAAGAGAGG	AACCGAGAGACCCTTTAACT	55.0	45.0	57.7	55.8	757-943 bp	1.0	70.6
trnS-trnG_p5	CCAACGCTTTAGTCCACTCA	AAACCGAGAGACCCTTTAACT	50.0	42.9	57.8	56.6	693-879 bp	1.0	70.0